mouse anti human cd70  (Bio-Rad)

Journal: iScience

Article Title: Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma

doi: 10.1016/j.isci.2023.108367

Figure Lengend Snippet: Characterization of ccRCC cancer cell-specific vocabulary within the TME (A) UMAP of the cancer cells from tumor samples. Two sub-clusters were identified at a resolution of 0.1: ccRCC1 (pink), and ccRCC2 (blue). Proportions of each sub-cluster among the 3 patients are represented later in discussion the UMAP. (B) Proportion of expressed genes belonging to the ribosomal gene family (in percentage), in the function of the total number of expressed genes, for each cancer cell subcluster. (C) Expression of CA9 (ccRCC cancer cell marker) for each cancer cell subcluster (left), and enrichment score of communication genes in each cancer cell subcluster (right). The scores were compared by Wilcoxon tests. (D) Functional enrichment analysis of ccRCC1 compared to ccRCC2 cells (left), or conversely (right). Genes differentially expressed between ccRCC1 and ccRCC2 cells and filtered for minimum log2FC of 0.5 and p < 0.05 were considered. See also Table S4 . (E) Expression of genes significantly upregulated by ccRCC2 compared two-by-two to all other non-tumoral cell clusters of the TME. Color corresponds to the intensity of average expression, and dot size to the percentage of cells expressing the gene in the cluster. (F) Expression of VEGFα, EGFR, TGFα, ANG, CD70, OPN, PLXNB2 by two ccRCC cell lines (786-O, Caki2), and a proximal tubule primary cell line (RPTEC), at protein levels. Expression of surface markers is quantified by flow cytometry using specific mean fluorescence intensity (MFI), corresponding to the MFI obtained with specific antibody subtracted by those with the corresponding isotype (n = 5 up to 7 biologically independent replicates). Secreted molecules were measured from cell lines supernatants (n = 8 biologically independent replicates). Data are represented as mean values ±SEM. Conditions were compared using Kruskal–Wallis statistical tests combined with a Dunn’s post-hoc. ns, not significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. See also Figure S2 .

Article Snippet: BUV737 Mouse anti-human CD70 (Clone Ki-24) , BD , Cat# 612856; RRID: AB_2739193.

Techniques: Expressing, Marker, Functional Assay, Flow Cytometry, Fluorescence

Journal: iScience

Article Title: Large-scale analysis of cell-cell communication reveals angiogenin-dependent tumor progression in clear cell renal cell carcinoma

doi: 10.1016/j.isci.2023.108367

Figure Lengend Snippet:

Article Snippet: BUV737 Mouse anti-human CD70 (Clone Ki-24) , BD , Cat# 612856; RRID: AB_2739193.

Techniques: Recombinant, Transfection, Luminex, Software, FCAP Assay

Journal: Oncoimmunology

Article Title: Targeting CD70 in combination with chemotherapy to enhance the anti-tumor immune effects in non-small cell lung cancer

doi: 10.1080/2162402X.2023.2192100

Figure Lengend Snippet: Viability and CD70 expression on NSCLC cell lines after treatment with chemotherapy three NSCLC cell lines (NCI-H1975, A549 and NCI-H1650) were treated with either vehicle (PBS) or docetaxel (IC 20 ) and cisplatin (IC 20 ). (a) Percentage (%) viable cells and (b) CD70 expression (gMFI) were assessed after 48 h. (c) CD70 expression was assessed on tumors of NCI-H1975-bearing Hu-NSG-IL15-CD34+ mice after treatment with vehicle (Pbs+nacl) or chemotherapy (DOC+CDDP), dissected at study endpoint. Experiments (A&B) were performed in triplicate. All data represent mean ± SEM. * P < 0.05.

Article Snippet: NSCLC cell lines were treated with docetaxel and cisplatin for 48 hours and stained with phycoerythrin (PE) mouse anti-human CD70 Abs (Clone Ki-24) or PE mouse IgG3 isotype control (eBioscience) to assess CD70 expression via flow cytometry.

Techniques: Expressing

Journal: Oncoimmunology

Article Title: Targeting CD70 in combination with chemotherapy to enhance the anti-tumor immune effects in non-small cell lung cancer

doi: 10.1080/2162402X.2023.2192100

Figure Lengend Snippet: Survival data and tumor kinetics after treatment with monotherapy or the combination of chemo-immunotherapy in the syngeneic LLC-bearing mouse model CD70+ LLC cells were subcutaneously injected in the left flank of C57BL/6 mice ( n = 62). After 10 days, when tumors reached an average size of 25–35 mm2, mice were randomized and divided over six different treatment groups; vehicle, chemotherapy (chemo), isotype (iso), mouse aCD70, sequential combination (SEQ) and simultaneous combination (SIM). (a) Treatment scheme showing timing of dosing for aCD70 or the corresponding isotype (10 mg/kg) with blue arrows and for chemotherapy (cisplatin, 1 mg/kg; docetaxel, 2.5 mg/kg) with gray arrows. (b) Survival and (c) tumor growth kinetics of LLC tumors for the different treatment regimens and (d) tumor volume at day 14 is shown. Data pooled from two independent experiments with n = 4–7 per condition per experiment. All data represent mean ± SEM. * P ˂ 0.05.

Article Snippet: NSCLC cell lines were treated with docetaxel and cisplatin for 48 hours and stained with phycoerythrin (PE) mouse anti-human CD70 Abs (Clone Ki-24) or PE mouse IgG3 isotype control (eBioscience) to assess CD70 expression via flow cytometry.

Techniques: Injection

Journal: BMC Biotechnology

Article Title: Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors

doi: 10.1186/1472-6750-4-16

Figure Lengend Snippet: Structure of retroviral constructs. TFG vectors are MFG-based retroviral plasmids into which the hCD70 cDNA and hCD80 cDNAs have been cloned, with either a neoR or a zeoR selectable marker gene. The three genes are co-translated from a tricistronic mRNA which was transcribed from the 5' long terminal repeat. The translation of the first cDNA (CD70 or CD80) depends on the 5' long terminal repeat-gag IRES, while the translation of the selectable marker and the second cDNA (CD70 or CD80) are dependent on two IRESes from different origins.

Article Snippet: Transduced cells were stained for membrane expression of the two co-stimulatory molecules using PE-conjugated mouse anti-human CD70 mAb and FITC-conjugated mouse anti-human CD80 mAb (Pharmingen, Hamburg, D) as previously described [ ].

Techniques: Construct, Clone Assay, Marker

Journal: BMC Biotechnology

Article Title: Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors

doi: 10.1186/1472-6750-4-16

Figure Lengend Snippet: Example of co-stimulatory molecule expression by melanoma cells after transduction with TFG tricistronic vector: Immunostaining with hCD80 –(x-axis) and hCD70 – (y-axis) 3A : non transduced melanoma cells were cultured. Ccells (3 × 10 5 ) was stained for surface expression of CD70 and CD80 using specific antibodies as described in the Methods . 3B-3E : Murine B16.F10 melanoma cells were transduced with the four TFG retroviral vectors. Forty eight hours after transduction, pools of cells (3 × 10 5 ) were stained for surface expression of CD70 and CD80 using specific antibodies as described in the Methods . 2B: TFG EMCV ZEO transduced cells, 2C: TFG c-MYC ZEO transduced cells, 2D: TFG FGF-2 ZEO transduced cells, 2E: TFG HTLV-1 ZEO transduced cells. 3F : Human melanoma cells were transduced with the TFG FGF-2 ZEO. A selected pool of cells (3 × 10 5 ) was stained for surface expression of CD70 and CD80 using specific antibodies as described in the Methods . The samples were subjected to two-color analysis by flow cytometry. Data are shown as immunofluorescence profiles with arbitrary fluorescence units (log) of FITC on the x-axis and immunofluorescence profiles with arbitrary fluorescence units (log) of PE on the y-axis. To evaluate the percentage of co-stimulatory molecules expressed by transfected cells, the marker was set to allow < 5% positive cells in the non-transduced cells.

Article Snippet: Transduced cells were stained for membrane expression of the two co-stimulatory molecules using PE-conjugated mouse anti-human CD70 mAb and FITC-conjugated mouse anti-human CD80 mAb (Pharmingen, Hamburg, D) as previously described [ ].

Techniques: Expressing, Transduction, Plasmid Preparation, Immunostaining, Cell Culture, Staining, Flow Cytometry, Immunofluorescence, Fluorescence, Transfection, Marker

Journal: BMC Biotechnology

Article Title: Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors

doi: 10.1186/1472-6750-4-16

Figure Lengend Snippet: Effects of CD70 and CD80 co-expression by single transduced tumor cells on tumor growth in an established model. 4A : 10 5 B16.F10 wt (control cells), or CD70 and CD80 double transduced B16.F10, or CD70 and CD80 single transduced B16.F10 were injected s.c. on day 0 into female C57BL/6 mice. 4B : 10 5 TS/A wt (control cells), or CD70 and CD80 double-transfected TS/A, or CD70 and CD80 single-transfected TS/A were injected s.c. on day 0 in female BALB/c mice. Tumor growth was monitored twice a week. The results are expressed as mean size (mm 2 ) of tumors from groups that each contain five mice ± SD. The data represent mean +/- SD of a single experiment representative of three.pouet

Article Snippet: Transduced cells were stained for membrane expression of the two co-stimulatory molecules using PE-conjugated mouse anti-human CD70 mAb and FITC-conjugated mouse anti-human CD80 mAb (Pharmingen, Hamburg, D) as previously described [ ].

Techniques: Expressing, Injection, Transfection